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A, Electrophoresis of whole serum (WS) or fractionated serum IgG derived from a systemic lupus erythematosus (SLE) patient (L) and a normal subject (N). Molecular weight marker (M) is shown at the right. B, Scatterplot of entire gene set considered by <t>microarray</t> analysis to be expressed in podocytes (P < 0.05). The position of each dot on the scatterplot corresponds to the normalized average signal intensity (log scale) of a single gene. The normalized average signal intensity after exposure to SLE IgG and normal IgG is shown on the x and y axes, respectively. Broken red lines represent normal IgG:SLE IgG ratios of 2.0 (top; 2-fold greater expression in normal IgG) and 0.5 (bottom; 2-fold greater expression in SLE IgG). Black broken line is the mean.
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microarray solution software package  (SAS institute)
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A, Electrophoresis of whole serum (WS) or fractionated serum IgG derived from a systemic lupus erythematosus (SLE) patient (L) and a normal subject (N). Molecular weight marker (M) is shown at the right. B, Scatterplot of entire gene set considered by <t>microarray</t> analysis to be expressed in podocytes (P < 0.05). The position of each dot on the scatterplot corresponds to the normalized average signal intensity (log scale) of a single gene. The normalized average signal intensity after exposure to SLE IgG and normal IgG is shown on the x and y axes, respectively. Broken red lines represent normal IgG:SLE IgG ratios of 2.0 (top; 2-fold greater expression in normal IgG) and 0.5 (bottom; 2-fold greater expression in SLE IgG). Black broken line is the mean.
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A, Electrophoresis of whole serum (WS) or fractionated serum IgG derived from a systemic lupus erythematosus (SLE) patient (L) and a normal subject (N). Molecular weight marker (M) is shown at the right. B, Scatterplot of entire gene set considered by microarray analysis to be expressed in podocytes (P < 0.05). The position of each dot on the scatterplot corresponds to the normalized average signal intensity (log scale) of a single gene. The normalized average signal intensity after exposure to SLE IgG and normal IgG is shown on the x and y axes, respectively. Broken red lines represent normal IgG:SLE IgG ratios of 2.0 (top; 2-fold greater expression in normal IgG) and 0.5 (bottom; 2-fold greater expression in SLE IgG). Black broken line is the mean.

Journal: Arthritis & rheumatology (Hoboken, N.J.)

Article Title: Lupus Nephritis IgG Induction of Calcium/Calmodulin-Dependent Protein Kinase IV Expression in Podocytes and Alteration of Their Function

doi: 10.1002/art.39499

Figure Lengend Snippet: A, Electrophoresis of whole serum (WS) or fractionated serum IgG derived from a systemic lupus erythematosus (SLE) patient (L) and a normal subject (N). Molecular weight marker (M) is shown at the right. B, Scatterplot of entire gene set considered by microarray analysis to be expressed in podocytes (P < 0.05). The position of each dot on the scatterplot corresponds to the normalized average signal intensity (log scale) of a single gene. The normalized average signal intensity after exposure to SLE IgG and normal IgG is shown on the x and y axes, respectively. Broken red lines represent normal IgG:SLE IgG ratios of 2.0 (top; 2-fold greater expression in normal IgG) and 0.5 (bottom; 2-fold greater expression in SLE IgG). Black broken line is the mean.

Article Snippet: Scan data images were analyzed using the Microarray Data Analysis Tool software package (Filgen), a comprehensive program that also produced the gene ontology and Z score reports.

Techniques: Electrophoresis, Derivative Assay, Molecular Weight, Marker, Microarray, Expressing